SOME QUESTIONS THAT CAME UP
1) genotypes: we assume that all genes that are not mentioned are wild-type. Wild-type bacteria have "+" alleles for all their genes (and are sensitive to antibiotics)
2) For us, all lacO[c] mutants prevent the LacI repressor from binding.
3) If nothing is mentioned, we assume that there is no glucose in the medium
4) For the extra questions (the ones that had sucrose involved): think about what happens when sucrose is broken down in the cell...
5) Midterm: how are they going to test all the recombinant DNA stuff? You may have to interpret the results of a set of restriction digestions and come up with the map of the plasmid, or a cloning procedure may be described to you and you may have to draw the final product, pretend to restriction digest it, pretend to run the digestion reactions on a gel and draw the result (banding patterns), or...I think Dr Beatty may be posting old midterm questions on the 335 website!
Have a good week-and don't mix up northerns and Southerns when you do problem set 3 (the last question is quite tricky-focus on the first part of the problem set first!)
Cheers
Pam
PS: practice questions can be found here
or by clicking on 'lac operon stuff' (it's under the 'archives and links' column) and following the instruction.
(sorry about the formatting-I am trying to fix it)
P.P.S.: ANSWERS TO EXTRA PRACTICE QUESTIONS WILL BE AVAILBALE OVER THE WEEKEND (FOR NOW I TRIED TO SUMMARIZE OUR ANSWERS TO PROBLEMS SET 2-below)
posted by pam39 @ 4:14 PM
4 Comments:
Hi Pam!
I was wondering for the extra tutorial questions that you put up today on the overhead. You said you posted it on the website. I can't find it!!!! Maybe it's because im new to this blog thing...sorry!!! Thanks a million!!!
Go to the left column, under 'archives and links". There's a link that says 'lac operon stuff'. Click on it and it should take you to a page with some lac operon info.
There's a menu bar on on that page...choose 'practice questions' and then follow the instructions, there are 3 lac questions and one 'winterose' operon question ready to use (some formatting got messed up in one of the questions...trying to fix it!)
Cheers
Pam
Thanks Pam! Now that I found the questions, I tried accessing them and for question 1 under lac operon stuff, it says that the snapshot im trying to access is private! I can't get through! But i can access question 2 and 3 fine. Thanks for the quick response!
Thanks for pointing that out!
They should work now.
Cheers,
Pam
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