BIOL335 LOUNGE

A place for BIOL335 people to ask, answer and discuss questions, place their 'requests' and make suggestions

Saturday, April 07, 2007

Anonymous said...: With regards to Linker Scanning Mutagenesis,

I understand that you can replace a portion of DNA with "junk DNA" by using restriction endonucleases. How do you use PCR to put in the junk DNA?

Thanks; 2:30 PM
Anonymous said...: Hey!

You would not use PCR to physically "put in junk DNA", but rather to replace given base pairs with other base pairs (same Idea as for PCR-based site directed mutagenesis). You could also generate a "random piece of DNA" with PCR, and then replace the original DNA with it using restriction enzymes.

Sorry about the confusion!; 3:53 PM