Questions about Dr Beatty's section
In an effort to be a bit more organized...
If you have any questions regarding Dr Beatty's section (prokaryotes stuff, restriction mapping, etc), please post them in this post's comments section.
(Thanks to the person who suggested this!)
posted by pam39 @ 10:35 AM
10 Comments:
Hi Pam,
I was wondering if it is possible for you to post a brief answer to Dr. Beatty's problem set 3 as you did for problem set 2.
Thanks aloott
Hi Pam
sorry if this is short notice..
but could you go over the significance of Kanr for 02 regulation?
Sure!
However, you'll need to expand yoour question a bit. Kan resistance per se is unrelated to O2 regulation, so you'll have to put your question in context...
I understand that Kanr is used to detect O2 regulation and signal transduction pathways...
is it because Kanr is used as a plasmid to detect levels with crt?
I am really sorry, but I think I need more info to answer this.
As far as I know, Kanr stands for "kanamycin resistance", which indicates a gene encoding a protein that confers resistance to the antibiotic kanamycin. This gene is routinely used as a selectable marker, for example to only allow those bacteria which received the plasmid (containing the Kanr) to grow on a media containing kanamycin.
Cheers
Pam
Does anyone know if problem set 3 is going to be on the final?
Hi everyone,
all I know is that you are much morelikely to be tested on something from AFTER the midterm.
Cheers
Pam
What are STS and how are they used to assemble physical maps?
STS= sequence tag sites
That's right. They are used just like VNTRs or RFLPs to assemble a contig map (physical map). If 2 clones have the same STS, then they must be overlapping. Each STS is a short, unique sequence in the genome, used as a molecular marker.
Happy studying!
Pam
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